Journal: Frontiers in Physiology

Article Title: In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos ( Anser cygnoides )

doi: 10.3389/fphys.2022.858274

Figure Lengend Snippet: In ovo injection of CHIR-99021 in goose embryos activates the Wnt signaling pathway by inhibiting GSK-3β. The expression of FZD4, β-catenin, LEF1, TCF4, and c-Myc in dorsal tissues of each group at E13 (A) , E17 (B) , E21 (C), and E25 (D) , as detected by western blotting. All data are shown as mean ± SEM, no common superscript letter within the same embryonic day means statistical difference ( p < 0.05) by one-way ANOVA with Duncan’s multiple-range test. n = 6 embryos.

Article Snippet: The antibodies identifiers and dilutions were as follows: rabbit anti-β-catenin antibody (Cat# bs-23663R, bioss, Beijing, China), rabbit anti-GSK-3 β antibody (Cat# bs-0023M, bioss), 1:2,000,1:2,000; rabbit anti-c-Myc antibody (Cat# bs-4963R, bioss), 1:2,000; rabbit anti-TCF4 antibody (Cat# bs-1280R, bioss), 1:2000; rabbit anti-LEF1 antibody (Cat# bs-1843R, bioss), 1:1,500; rabbit anti-FZD4 antibody (Cat# bs-13217R, bioss), 1:2,000; ACTB rabbit monoclonal antibody (Cat# AC026, Abclonal, Wuhan, China), 1:50,000.

Techniques: In Ovo, Injection, Expressing, Western Blot

Journal: Mediators of Inflammation

Article Title: Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells

doi: 10.1155/2017/8379256

Figure Lengend Snippet: Western blot protein expression analysis of FZD4 (60 kDa), FZD6 (75 kDa), phospho- (86 kDa) and holo- β -catenin (96 kDa), and MMP7 (28 kDa) in decidualized St-T1 and KdS1. (a) shows the protein expressions of FZD4 in dSt-T1 and (b) in dKdS1, (c) of FZD6 in dSt-T1 and (d) in dKdS1, (e) of β -catenin and phospho- β -catenin, and (f) of MMP7 and (g) NF κ B signaling after incubation with 0.1 ng/mL IL-1 β for 0 and 48 h in representative western blot analysis and the corresponding pixel density evaluation (omitted for MMP7, p65, and I κ B; TNF α was used as control for NF κ B activity). Data represents means ± SD with ∗ p < 0.05 for IL-1 β treated cells versus untreated cells. β -Actin analysis served as control.

Article Snippet: The following primary antibodies were used in this study: rabbit monoclonal anti-human p44/42 MAPK (ERK1/2, 42 and 44 kDa, 1 : 2000 dilution), rabbit monoclonal anti-human phospho-p44/42 MAPK (phospho-ERK1/2 (Thr202/Tyr204) (pERK), 42 and 44 kDa, 1 : 2000 dilution), rabbit monoclonal anti-human c-Jun (43 and 48 kDa, 1 : 500 dilution), rabbit monoclonal anti-human phospho-c-Jun ((Ser73) (pc-Jun), 48 kDa, 1 : 500 dilution, all Cell Signaling Technology, MA, USA), rabbit polyclonal anti-human Sdc-1 (34 kDa, 1 : 1000 dilution, Abcam), rabbit polyclonal anti-frizzled 4 (60 kDa, 1 : 2000 dilution, Abcam), rabbit monoclonal anti-frizzled 6 (79 kDa, 1 : 500 dilution, Cell Signaling), rabbit polyclonal anti-beta-catenin (96 kDa, 1 : 1000 dilution, Abcam), rabbit polyclonal anti-phospho-beta-catenin (86 kDa, 1 : 800 dilution, Abcam), rabbit polyclonal anti-MMP7 (28 kDa, 1 : 500 dilution, Abcam), rabbit monoclonal anti-NF κ B p65 (65 kDa, 1 : 1000 dilution), rabbit monoclonal anti-phospho NF κ B pp65 (65 kDa, 1 : 1000 dilution), and mouse monoclonal anti-I κ B α (39 kDa, 1 : 1000 dilution, all Cell Signaling).

Techniques: Western Blot, Expressing, Incubation, Activity Assay